Beneficial effects of linagliptin in cell culture model of Parkinson’s disease

Objectives: We aimed to investigate the neuroprotective effects of linagliptin in an vitro 6-hydroxydopamine (6-OHDA) Parkinson’s disease model. Methods: 6-OHDA (200 µM) were administered SH-SY5Y cells for 24 h induce model vitro. Cells treated with (1, 10, 50 and 100 nM) 30 minutes before administration. Cell viability was examined by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method lactate dehydrogenase (LDH) analysis. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) reactive oxygen species (ROS) analyses conducted assess oxidative stress. Apoptosis evaluated Caspase-3 mRNA expression levels. Results: It observed that elevated LDH levels cell death. Oxidative stress exaggerated increased ROS MDA substantially apoptosis proven cells. Pretreatment alleviated apoptosis. Conclusions: Given its role as well on apoptosis, may be a drug candidate Parkinson's disease.